| Инд. авторы: | Romashchenko A.V., Kan T.-W., Petrovski D.V., Gerlinskaya L.A., Moshkin M.P., Moshkin Y.M. |
| Заглавие: | Nanoparticles Associate with Intrinsically Disordered RNA-Binding Proteins |
| Библ. ссылка: | Romashchenko A.V., Kan T.-W., Petrovski D.V., Gerlinskaya L.A., Moshkin M.P., Moshkin Y.M. Nanoparticles Associate with Intrinsically Disordered RNA-Binding Proteins // ACS Nano. - 2017. - Vol.11. - Iss. 2. - P.1328-1339. - ISSN 1936-0851 . - EISSN 1936-086X. |
| Внешние системы: | DOI: 10.1021/acsnano.6b05992; РИНЦ: 29491502; PubMed: 28122180; SCOPUS: 2-s2.0-85014253041; WoS: 000395357300024; |
| Реферат: | eng: Nanoparticles are capable of penetrating cells, but little is known about the way they interact with intracellular proteome. Here we show that inorganic nanoparticles associate with low-complexity, intrinsically disordered proteins from HeLa cytosolic protein extracts in nondenaturing in vitro nanoparticle pull-down assays. Intrinsic protein disorder associates with structural mobility, suggesting that side-chain flexibility plays an important role in the driving of a protein to nanoparticle absorption. Disordered protein domains are often found in a diverse group of RNA-binding proteins. Consequently, the nanoparticle-associated proteomes were enriched in subunits of RNA-processing protein complexes. In turn, this indicates that within a cell, nanoparticles might interfere with protein synthesis triggering a range of cellular responses. © 2017 American Chemical Society.
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| Ключевые слова: | proteomics; RNA-binding proteins; stress granules; Bins; Biosynthesis; Molecular biology; Nanoparticles; RNA; Synthesis (chemical); intrinsically disordered proteins; Disordered proteins; Inorganic nanoparticle; Intrinsically disordered proteins; Protein complexes; Proteomics; RNA-binding protein; Structural mobility; Proteins; Cytosolic proteins; nanoparticles; |
| Издано: | 2017 |
| Физ. характеристика: | с.1328-1339 |
