| Инд. авторы: | Volkova O.A., Kondrakhin Y.V., Yevshin I.S., Valeev T.F., Sharipov R.N. |
| Заглавие: | Assessment of translational importance of mammalian mRNA sequence features based on Ribo-Seq and mRNA-Seq data |
| Библ. ссылка: | Volkova O.A., Kondrakhin Y.V., Yevshin I.S., Valeev T.F., Sharipov R.N. Assessment of translational importance of mammalian mRNA sequence features based on Ribo-Seq and mRNA-Seq data // Journal of Bioinformatics and Computational Biology. - 2016. - Vol.14. - Iss. 2. - Art.1641006. - ISSN 0219-7200. - EISSN 1757-6334. |
| Внешние системы: | DOI: 10.1142/S0219720016410067; РИНЦ: 27097374; PubMed: 27122318; SCOPUS: 2-s2.0-84971639725; WoS: 000376653200008; |
| Реферат: | eng: Ribosome profiling technology (Ribo-Seq) allowed to highlight more details of mRNA translation in cell and get additional information on importance of mRNA sequence features for this process. Application of translation inhibitors like harringtonine and cycloheximide along with mRNA-Seq technique helped to assess such important characteristic as translation efficiency. We assessed the translational importance of features of mRNA sequences with the help of statistical analysis of Ribo-Seq and mRNA-Seq data. Translationally important features known from literature as well as proposed by the authors were used in analysis. Such comparisons as protein coding versus non-coding RNAs and high-versus low-translated mRNAs were performed. We revealed a set of features that allowed to discriminate the compared categories of RNA. Significant relationships between mRNA features and efficiency of translation were also established.
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| Ключевые слова: | ALIGNMENT; CONSENSUS; EVOLUTIONARY; TRANSCRIPTION; LENGTH; EFFICIENCY; INITIATION; NONCODING RNAS; PROTEIN-SYNTHESIS; discriminant analysis; regression analysis; lncRNAs; mRNA-Seq; Ribo-Seq; ribosome profiling; translation efficiency; Mammalian mRNAs; DYNAMICS; |
| Издано: | 2016 |
| Физ. характеристика: | 1641006 |
